Expression in E.Coli and Antigenicity of HGV NS5 Protein
- VernacularTitle:庚型肝炎病毒NS5 cDNA片段的表达及其免疫原性的研究
- Author:
Rui, GONG
;
Hui-wen, MA
;
Li-heng, TONG
- Publication Type:Journal Article
- From:Virologica Sinica
2001;16(2):114-118
- CountryChina
- Language:Chinese
-
Abstract:
A 880 bp cDNA localized to the putative NS5 region of HGV genome was expressed in E.coli BL21(DE3). The cDNA fragment was inserted into a plasmid pGEX-5X-1,at the downstream of the DNA sequence encoding Schistosoma japonicum glutathione S- transferase(GST),in the same reading frame with the gene of GST. A 60KD GST-NS53 fusion protein was expressed at 37℃ in a form of inclusion bodies amounting to 30 percent of total host protein whereas at 20℃ mainly in a form of soluble protein . The fusion protein was extracted and purified to homologue. The purified GST-NS53 fusion protein could be specifically recognized with either the sera from the patient infected by HGV or the antisera directed against GST.
