Studies on Apoptosis of Host Cells Induced by Foot-and-Mouth Disease Virus
- VernacularTitle:口蹄疫病毒诱导宿主细胞凋亡的研究
- Author:
Hai-xiang, WU
;
Cong-yi, ZHENG
;
San-fu, QU
;
Jing-zhe, GUO
;
Yan-li, WANG
- Publication Type:Journal Article
- From:Virologica Sinica
2001;16(2):175-178
- CountryChina
- Language:Chinese
-
Abstract:
Apoptosis of PK-15 cells induced by Foot-and-Mouth Disease Virus (FMDV) in vitro was reported in this paper. Typical cell apoptosis was detected by use of Hoechst 33258 fluorescence probe, agarose gel electrophoresis and in situ end-labeling (TUNEL). After PK-15 cells were infected by titration of 4.8 lg TCID50/mL FMDV for 32 h, apoptosis characteristics of nuclear condensation, fragmentation, accompanied by apoptotic bodies formation (Hoechst 33258 staining), 180-200 integer-fold sized pieces DNA Ladders (agarose gel electrophoresis) and strong green fluorescence dots (TUNEL) were all exhibited, and cell apoptosis was approximately 20%. In addition, the quantitative analysis of apoptosis in PK-15 cells induced by FMDV showed that apoptosis was correlated with infection of virus, and it was also time-dependent. Results indicate that FMDV can induce apoptosis of host cells and apoptosis plays an important role in the cytopathogencity effect of FMDV.
