Immunoreactivity Studies of Various HEV Antigen with Anti-HEV IgM of the Sear from Patients Infected with Hepatitis E Virus
- VernacularTitle:不同戊肝抗原检测抗-HEV IgM反应性研究
- Author:
Guang-ya, RONG
;
Ji-wen, ZHOU
;
Li, LEI
;
Gui-lan, ZHAO
;
Jie, SUN
- Publication Type:Journal Article
- From:Virologica Sinica
2001;16(2):128-130
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the Immunoreactivity of various HEV Antigen with Anti-HEV IgM. Methods Solid-phase enzyme immunoassay( EIA ) was developed for detecting anti-HEV IgM by using synthetic peptides E30, E42, E33, and recombinant antigen from HEV ORF-2. Results Of 60 anti-HEV positive sera by using E30, E42, E33 and recombinant antigen as coating antigen, Anti-HEV IgM positive rates were 76.7%, 26.6%, 18.3% and 66.7% respectively. In Acute-phase and convalescence-phase sera of the patients with Hepatitis E, Anti-HEV IgM positive rate was 90% and 3.3% respectively. Conclusions The HEV E30-based EIA will be very useful in the early diagnosis of Hepatitis E.
