Rapid detection of molecular variation of Rice stripe virus in China by PCR-SSCP assay
- VernacularTitle:应用PCR-SSCP技术快速检测我国水稻条纹病毒的分子变异
- Author:
Han-xin, LIN
;
Tai-yun, WEI
;
Zu-jian, WU
;
Qi-ying, LIN
;
Lian-hui, XIE
- Publication Type:Journal Article
- From:Virologica Sinica
2001;16(2):166-169
- CountryChina
- Language:Chinese
-
Abstract:
A reverse transcription polymerase chain reaction (RT-PCR) and single-strand conformation polymorphisms (SSCP) assay were applied to rapidly detect the molecular variability in CP and SP genes among seven isolates of Rice stripe virus in China. The PCR results showed that the CP gene of JD isolate and SP gene of PJ isolate could not be amplified. SSCP analysis showed that there were completely different electrophoretic pattern of CP gene among six isolates. To SP gene, SSCP results also discovered polymorphisms. There were five patterns among these isolates, and the pattern of YL and BS isolates were same.
