Heme oxygenase-1 expression in rats with acute lung rejection and implication.
- Author:
Ke, JIANG
;
Lin, CHENG
;
Jiangjun, WANG
;
Jinsong, LI
;
Jun, NIE
- Publication Type:Journal Article
- MeSH:
Graft Rejection/*enzymology;
Heme Oxygenase (Decyclizing)/genetics;
Heme Oxygenase (Decyclizing)/*metabolism;
Lung Transplantation;
RNA, Messenger/genetics;
RNA, Messenger/metabolism;
Rats, Sprague-Dawley;
Rats, Wistar
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2009;29(1):84-7
- CountryChina
- Language:English
-
Abstract:
This study investigated the expression of hemeoxygenase-1 (HO-1) in rats with acute lung rejection and its implication. A valid rat orthotopic left lung transplantation model (SD rat-->Wistar rat) was established by using an improved three-cuff anastomosis technique. The rats were divided into control group, CoPP (HO-1 inducer)-treated group and ZnPP (HO-1 inhibitor)- treated group. The severity of acute rejection was graded on the basis of the morphologic changes of the lung samples stained with HE. The expression of HO-1 protein in lung tissue was detected by using immunohistochemistry and Western blot, and HO-1 mRNA activity was assayed by RT-PCR. The results showed that the expression of HO-1 protein was significantly increased with the acute rejection grading in rats (P<0.01). As compared with control and ZnPP-treated groups, the severity of acute rejection was not alleviated and the grade not reduced significantly in CoPP-treated group (P>0.05). It was concluded that HO-1 protein might be involved in the pathological process of post-graft acute rejection. The expression of HO-1 protein was increased gradually with aggravation of acute rejection, and HO-1 protein might be used as an index to monitor acute rejection after lung transplantation.
