Tea polyphenols exerts anti-hepatitis B virus effects in a stably HBV-transfected cell line.
- Author:
Pian, YE
;
Shuling, ZHANG
;
Lei, ZHAO
;
Jihua, DONG
;
Shenghua, JIE
;
Ran, PANG
;
Shuli, LI
- Publication Type:Journal Article
- MeSH:
Antiviral Agents/*pharmacology;
DNA, Viral/analysis;
Dose-Response Relationship, Drug;
Flavonoids/*pharmacology;
Hep G2 Cells;
Hepatitis B Surface Antigens/analysis;
Hepatitis B e Antigens/analysis;
Hepatitis B virus/*drug effects;
Inhibitory Concentration 50;
Phenols/*pharmacology;
Tea/*chemistry
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2009;29(2):169-72
- CountryChina
- Language:English
-
Abstract:
In this study, the anti-HBV effects of tea polyphenols (TP) were examined. After cells were exposed to TP for 3, 6, 9 days, amounts of HBsAg, HBeAg and HBV-DNA released into the supernatant of the cultured HepG2 2.2.15 cells were detected. TP, to some extent, inhibited the secretion of HBsAg and strongly suppressed the secretion of HBeAg in a dose-dependent (P<0.01) and time-dependent manner, with 50% maximal inhibitory concentration (IC50) value being 7.34 microg/mL on the 9th day, but the time-dependence was not significant (P=0.051). Expression of HBV-DNA in the supernatant of the cell culture also was significantly decreased in a dose-dependent fashion (P<0.01). The IC50 of TP in inhibiting HBV DNA was 2.54 microg/mL. It concluded that TP possessed potential anti-HBV effects and may be used as a treatment alternative for HBV infection.
