Effects of deep brain stimulation on expression of DARPP-32 and its phosphorylated proteins in corpus striatum of rats with dyskinesia
- VernacularTitle:脑深部电刺激对异动症大鼠纹状体DARPP-32及其磷酸化蛋白表达的影响
- Author:
Zheng, PU
;
Li-xia, LU
;
Zhen-guo, LIU
- Publication Type:Journal Article
- Keywords:
Parkinson's disease;
dyskinesia;
deep brain stimulation;
subthalamic nucleus;
dopamine and adenosine 3'5'-monophosphate-regulated phosphor-protein
- From:Journal of Shanghai Jiaotong University(Medical Science)
2009;29(7):817-820
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of subthalamic nucleus (STN)-deep brain stimulation (DBS) on expression of dopamine and adenosine 3'5'-monophosphate-regulated phosphor-protein(DARPP-32) and its phosphorylated proteins in corpus striatum of rat models with levodopa-induced dyskinesia. Methods The rat models of levodopa-induced dyskinesia were set up and were given STN-DBS (stimulation group). The expression of DARPP-32 and its phosphorylated proteins in corpus striatum (damage side and normal side) were detected and compared with sham-stimulation group and sham-operation group. Results There was no significant difference in the expression of DARPP-32 total protein in corpus striatum of rats with dyskinesia among three groups (P>0.05). The expression of Phosphor-Thr34-DARPP-32 protein in the damage side of corpus striatum in stimulation group was significantly lower than that in sham-stimulation group and sham-operation group (P<0.05), while the expression of Phosphor-Thr75-DARPP-32 protein in the damage side of corpus striatum in stimulation group was significantly higher than that in sham-stimulation group and sham-operation group (P<0.05). Conclusion DARPP-32 and its phosphorylated proteins play an important role in the pathogenesis of levodopa-induced dyskinesia.
