siRNA specific to Pdx-1 disturbed the formation of the islet in early zebrafish embryos.
- Author:
Shen, CHEN
;
Jintao, HUANG
;
Guangming, YUAN
- Publication Type:Journal Article
- MeSH:
Embryo, Nonmammalian;
Homeodomain Proteins/genetics;
Homeodomain Proteins/*metabolism;
Islets of Langerhans/cytology;
Islets of Langerhans/*embryology;
Islets of Langerhans/metabolism;
RNA Interference;
RNA, Small Interfering/*genetics;
Trans-Activators/genetics;
Trans-Activators/*metabolism;
Zebrafish
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2007;27(6):639-42
- CountryChina
- Language:English
-
Abstract:
Pdx-1, an important transcription factor highlighting in the early pancreatic development, islet functions and pancreatic disorders, needs to be more investigated in zebrafish, and siRNA is still seldom applied in zebrafish embryo-related research. Our aim was to explore the role of pdx-1 in pancreatic development of zebrafish embryos by using siRNA approach. Microinjection, reverse transcriptase-PCR (RT-PCR), in situ hybridization and immunofluorescent staining were used in this research, and the morphology of the islet in normal zebrafish embryos, and in those treated with the siRNA specific to pdx-1 (siPDX-1) or siGFP was observed and compared. The expression of pdx-1 was detected in the stages of 1-cell, 2-cell, 4-cell, 8-cell, 16-cell, 16-hour by RT-PCT. The in situ hybridization and immunofluorescent staining results showed that siPDX-1 disturbed the formation of the islet in zebrafish embryos. Pdx-1 played multiple roles in maintaining the phenotype of the islet during embryogenesis in zebrafish.
