The expression of endothelin receptor B in melanoma cells A375 and Sk-mel-1 and the proliferative effects of endothelin 3 on A375 cells.
- Author:
Nengxing, LIN
;
Changzheng, HUANG
;
Jin, TIAN
;
Juan, TAO
;
Jin, ZHANG
;
Lingyun, YANG
;
Yan, LI
;
Yeqiang, LIU
;
Siyuan, CHEN
;
Guanxin, SHEN
;
Jiawen, LI
;
Chunsen, WANG
;
Yating, TU
- Publication Type:Journal Article
- MeSH:
Cell Line, Tumor;
Cell Proliferation/*drug effects;
Endothelin-3/*pharmacology;
Melanoma/*metabolism;
Melanoma/*pathology;
Receptor, Endothelin B/*metabolism;
Reverse Transcriptase Polymerase Chain Reaction
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2007;27(5):611-3
- CountryChina
- Language:English
-
Abstract:
In order to investigate the expression of endothelin receptor B (ETR-B) in human malignant melanoma (MM) cells A375 and SK-mel-1 and the proliferative effects of endothelin 3 (ET3) on A375 cells, RT-PCR was applied to detect the expression of ETR-B gene in human MM cells A375 and SK-mel-1. MTT method was used to evaluate the growth enhancing effects of ET3 on A375 cell line in vitro. The results showed that ETR-B gene was expressed in both MM A375 and SK-mel-1 cells. ET3 had stronger ability to enhance the proliferation of A375 cells in vitro in a concentration-dependent manner. It was suggested that ET3/ETR-B might play an important proliferative role in MM.
