Effect of Stathmin decoy-oligodeoxynucleotides on the proliferation and differentiation of precartilainous stem cells.
- Author:
Fengjing, GUO
;
Yibei, ZHANG
;
Anmin, CHEN
- Publication Type:Journal Article
- MeSH:
Cartilage/*cytology;
Cell Differentiation/*drug effects;
Cell Proliferation/drug effects;
Cells, Cultured;
Oligodeoxyribonucleotides/genetics;
Oligodeoxyribonucleotides/*pharmacology;
Rats, Sprague-Dawley;
Stathmin/*genetics;
Stathmin/pharmacology;
Stem Cells/*cytology
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2007;27(5):557-60
- CountryChina
- Language:English
-
Abstract:
By using decoy-oligodeoxynucleotides (decoy-ODNS) technique, the effects of Stathmin gene on the proliferation and differentiation of in vitro cultured precartilainous stem cells (PSCs) were investigated. The Stathmin decoy-ODNs were transfected into PSCs in rats by using gene transfection technique. Under the induction of cortisol (1 micromol/L), electrophoretic mobility shift assay was used the inhibitory effects of decoy-ODNS on Stathmin gene. MTT and cytometry were used to test the cell proliferation. The expression of collagen II and V and Stathmin protein was detected by using Western blot. The results showed that Stathmin decoy-ODNs inhibited the Stathmin activity in a dose-dependent manner. When the concentration of decoy-ODNs was 10 times of standard concentration, the proliferation of PSCs was obviously suppressed and the differentiation happened. Compared to the control group, the difference was significant (P<0.05). It was concluded that decoy-ODNs could inhibit the proliferation and promote the differentiation of PSCs by antagonizing Stathmin activity.
