Effect of anti-KDR antibody on the proliferation of hemangioma vascular endothelial cells in vitro.
- Author:
Yiqing, LI
;
Yin, XIA
;
Bi, JIN
- Publication Type:Journal Article
- MeSH:
Antibodies/*pharmacology;
Cell Proliferation/drug effects;
Endothelium, Vascular/*pathology;
Hemangioma/*pathology;
Tumor Cells, Cultured;
Vascular Endothelial Growth Factor Receptor-2/*immunology
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2007;27(5):551-3
- CountryChina
- Language:English
-
Abstract:
The suppressive effect of anti-KDR antibody against VEGF on proliferation of hemangioma-derived vascular endothelial cells (HVECs) was investigated. HVECs from one case of hemangioma in proliferative phase were cultured. Both primary culture and sub-culture were conducted in M199 medium. The HVECs of passage 3 were divided into 4 groups based on the concentrations of anti-KDR antibody. Cell count was performed and inhibitory rate of HVECs was measured before and 9 days after interference. The results showed that the number of HVECs in the anti-KDR antibody-treated groups was significantly decreased and the inhibitory rate of HVECs by anti-KDR antibody (50, 10 and 2 microg/mL) was 84%, 63% and 39% respectively at 9th day after interference, with the difference being significant. In the control group, the number of HVECs was increased significantly. In was concluded that the anti-KDR antibody could suppress the activity of VEGF through blocking the KDR, indicating the potential clinical applications of anti-KDR antibody in the treatment of hemangioma.
