Protective effects of oxymatrine on adriamycin-induced cardiotoxicity in rabbits and its mechanism
- VernacularTitle:氧化苦参碱对兔阿霉素心肌损伤保护作用及其机制研究
- Author:
Fei, MA
;
Xiao-ping, LI
;
Jian-chun, GU
;
Li, ZHANG
;
Lei-zheng, ZHENG
- Publication Type:Journal Article
- Keywords:
adriamycin;
cardiotoxicity;
oxymatrine;
apoptosis;
superoxide dismutase;
glutatione peroxidase
- From:Journal of Shanghai Jiaotong University(Medical Science)
2009;29(6):685-688
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the in vivo models of adriamycin(ADR)-induced cardiotoxicity in rabbits, investigate the protective effect of oxymatrine (OMT) on ADR-induced cardiotoxicity, and explore the possible mechanism. Methods Twenty-six rabbits were randomly divided into ADR group (n=8, 2 mg/kg ADR), OMT group (n=5, 10 mg/kg OMT), ADR + OMT group (n=8, 10 mg/kg OMT was injected 30 min before ADR injection) and saline group (n=5, same quantity of normal saline), and rabbits in each group were infused with medicine or normal saline through ear marginal vein once a week for 8 weeks. The apoptosis of myocardial cells was detected by TUNEL methods, and the activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) was determined. Results After treatment, the body weight of ADR group was significantly lower than that of the other groups(P < 0.05), the activity of SOD and GSH-Px significantly decreased and the apoptosis index (AI) was significantly higher than that of the other groups(P <0.01). There were similar while minor changes in ADR + OMT group. There was no significant adverse effects in OMT group. Conclusion OMT protects heart from adriamycin-induced injury in rabbits, which may relate to the decrease in level of antioxidant and apoptosis of myocardial cells.
