High glucose promotes the CTGF expression in human mesangial cells via serum and glucocorticoid-induced kinase 1 pathway.
- Author:
Quansheng, WANG
;
Ali, ZHANG
;
Renkang, LI
;
Jianguo, LIU
;
Jiwen, XIE
;
Anguo, DENG
;
Yuxi, FENG
;
Zhonghua, ZHU
- Publication Type:Journal Article
- MeSH:
Cells, Cultured;
Connective Tissue Growth Factor/genetics;
Connective Tissue Growth Factor/*metabolism;
Glucose/*pharmacology;
Immediate-Early Proteins/metabolism;
Immediate-Early Proteins/*physiology;
Mesangial Cells/cytology;
Mesangial Cells/*metabolism;
Protein-Serine-Threonine Kinases/metabolism;
Protein-Serine-Threonine Kinases/*physiology;
Signal Transduction/drug effects
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2008;28(5):508-12
- CountryChina
- Language:English
-
Abstract:
The role of serum and glucocorticoid-induced kinase 1 (SGK1) pathway in the connective tissue growth factor (CTGF) expression was investigated in cultured human mesangial cells (HMCs) under high glucose. By using RT-PCR and Western blot, the effect of SGK1 on the CTGF expression in HMCs under high glucose was examined. Overexpression of active SGK1 in HMCs transfected with pIRES2-EGFP-S422D hSGK1 (SD) could increase the expression of phosphorylated SGK1 and CTGF as compared with HMCs groups transfected with pIRES2-EGFP (FP) under high glucose or normal glucose. Overexpression of inactive SGK1 in HMCs transfected with pIRES2-EGFP-K127N hSGK1 (KN) could decrease phosphorylated SGK1 and CTGF expression as compared with HMCs groups transfected with FP under high glucose. In conclusion, these results suggest that high glucose-induced CTGF expression is mediated through the active SGK1 in HMCs.
