Apoptotic sensitivity to irradiation increased after transfection of chk1 antisense chain to HL-60 cell line.
- Author:
Yi, TANG
;
Wenli, LIU
;
Jianfeng, ZHOU
;
Qinglei, GAO
;
Jianhong, WU
- Publication Type:Journal Article
- MeSH:
*Apoptosis/radiation effects;
Cell Cycle/radiation effects;
HL-60 Cells;
Oligonucleotides, Antisense/*genetics;
Protein Kinases/*genetics;
Protein Kinases/metabolism;
Radiation Tolerance/*genetics;
Transfection
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2005;25(5):513-5
- CountryChina
- Language:English
-
Abstract:
The HL-60 cells were transfected with chk1 antisense and sense chain, and 24 h later subjected to irradiation. Twenty-four h after irradiation, the changes in the chk1 protein expression was assayed by Western blot, and the cell cycles and apoptosis rate detected by FCM. The irradiated apoptosis sensitivity was increased by antisense blocking of chk1 gene in HL-60 cell line with the apoptosis rate being 26.31%, significantly higher than that by the sense blocking (10.34%, 0.025 < P < 0.05). In HL-60 cells transfected with chk1 antisense chain, the G2/M phase arrest was attenuated and the cells in G2/M phase were accounted for 38.42%, significantly lower than those of the cells transfected with chkl sense chain (54.64%, 0.005 < P < 0.01). It was concluded that antisense blocking of chk1 gene could increase the apoptosis sensitivity to irradiation.
