Investigation of Early Protein Changes in the Urinary Bladder Following Partial Bladder Outlet Obstruction by Proteomic Approach.
10.3346/jkms.2005.20.6.1000
- Author:
Hyung Jee KIM
1
;
Inho SOHNG
;
Dong Hee KIM
;
Dong Cho LEE
;
Cheol Ho HWANG
;
Ji Young PARK
;
Jin Woo RYU
Author Information
1. The Proteomics Research Group, Department of Urology, Dankook University College of Medicine, Cheonan, Korea. killtumor@dankook.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Bladder;
Prostatic Hyperplasia;
Proteomics;
Urethral Obstruction
- MeSH:
Animals;
Bladder/*metabolism;
Bladder Neck Obstruction/genetics/*metabolism;
Down-Regulation;
Electrophoresis, Gel, Two-Dimensional;
Female;
Gene Expression;
Proteins/genetics/isolation and purification/*metabolism;
Proteomics;
Rats;
Rats, Sprague-Dawley;
Research Support, Non-U.S. Gov't;
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization;
Up-Regulation
- From:Journal of Korean Medical Science
2005;20(6):1000-1005
- CountryRepublic of Korea
- Language:English
-
Abstract:
We investigated the pathophysiological mechanism by proteomic approach as a possible tool to detect the marker proteins to develop lower urinary tract symptoms following bladder outlet obstruction (BOO). Rats were randomized into 3 groups; control, sham operation and BOO groups. BOO group was divided into 1, 3, and 5 day-group. Conventional proteomics was performed with high resolution 2-D gel electrophoresis followed by computational image analysis and protein identification using mass spectrometry using rat urinary bladders. A comparison of bladder of BOO group with control bladder showed that three proteins of optineurin, thioredoxin and preprohaptoglobin were over-expressed in the bladder of BOO group. In addition, four proteins, such as peroxiredoxin 2, transgelin, hippocampal cholinergic neurostimulating peptide (HCNP) and beta-galactoside-binding lectin, were under-expressed in the bladder of BOO group. These data supported that downregulation of HCNP might make detrusor muscle be supersensitive to acetylcholine, up-regulation of optineurin means the protection of nerve injury, and down-regulation of transgelin means the decreased contractility of detrusor muscle. Beside these proteins, other proteins are related to oxidative stress or have a nonspecific function in this study. However more information is needed in human bladder tissue for clinical usage.
