Cytokine Production by Whole Blood Cells: Relationship to Interleukin Gene Polymorphism and Bone Mass.
10.3346/jkms.2005.20.6.1017
- Author:
Jung Gu KIM
1
;
Seung Yup KU
;
Kyung Sil LIM
;
Byung Chul JEE
;
Chang Suk SUH
;
Seok Hyun KIM
;
Young Min CHOI
;
Shin Yong MOON
Author Information
1. Department of Obstetrics and Gynecology, College of Medicine, Seoul National University, Seoul, Korea. kimjg@plaza.snu.ac.kr
- Publication Type:Original Article ; In Vitro ; Research Support, Non-U.S. Gov't
- Keywords:
Interleukins;
Polymorphism, Genetic;
Bone Demineralization, Pathologic;
Blood Cells
- MeSH:
Aged;
Blood Cells/drug effects/immunology;
Bone Density/*genetics/*immunology;
Bone Diseases, Metabolic/blood/genetics/immunology;
Cytokines/*biosynthesis/blood;
Female;
Humans;
In Vitro;
Interleukin-1/biosynthesis/blood/genetics;
Interleukin-6/biosynthesis/blood/genetics;
Interleukins/*genetics;
Lipopolysaccharides/pharmacology;
Middle Aged;
Osteoporosis, Postmenopausal/blood/genetics/immunology;
*Polymorphism, Genetic;
Receptors, Interleukin-6/biosynthesis/blood/genetics;
Research Support, Non-U.S. Gov't;
Sialoglycoproteins/biosynthesis/blood/genetics
- From:Journal of Korean Medical Science
2005;20(6):1017-1022
- CountryRepublic of Korea
- Language:English
-
Abstract:
The aims of this study were to investigate the relationships between the production of interleukin-1 (IL-1), and IL-6 system by whole blood cells, and bone mineral density (BMD), and polymorphisms in IL-1 system and IL-6 gene in postmenopausal Korean women. The production of IL-1alpha, IL-1beta, IL-1 receptor antagonist (IL-1ra), IL-6, and soluble IL-6 receptor (sIL-6r) by lipopolysaccharide-stimulated whole blood cells was measured by ELISA in 110 subjects. Serum osteocalcin, C-telopeptide of type I collagen, and BMD at lumbar spine and proximal femur were measured. IL-1alphaC(-889)T polymorphism, IL-1beta C(-511)T polymorphism, 86-base pair variable number tandem repeat polymorphism in the IL-1ra gene, and IL-6 C(-634)G polymorphism were analyzed. The production of IL-1beta correlated positively with BMD at femoral neck, whereas the production of other ILs did not correlate with BMD at the skeletal sites examined. No significant differences in the production of ILs were observed among normal, osteopenic and osteoporotic postmenopausal women, and among the different IL system polymorphisms groups studied. No correlation between bone turnover markers and the production of ILs was noted. In conclusion IL-1beta may regulate bone metabolism at femoral neck, and the IL system polymorphism do not affect the production of ILs by whole blood cells.
