Interaction among Rb/p16, Rb/E2F1 and HDAC1 proteins in gallbladder carcinoma.

Author: Xin, WANG ; Kai, HUANG ; Lining, XU
Publication Type:Journal Article
From: Journal of Huazhong University of Science and Technology (Medical Sciences) 2009;29(6):729-31
CountryChina
Language:English
Abstract: The mechanism and interaction among Rb/p16, Rb/E2F1 and HDAC1 proteins in gallbladder carcinoma were investigated. By using the immunoprecipitation method, the interactions among Rb, p16, E2F1, HDAC1 proteins in gallbladder carcinoma cell line (Mz-ChA-1) were studied. It was found that there were Rb and E2F1 proteins in the precipitates with anti-HDAC1, and there were HDAC1 and E2F1 proteins in the precipitate with anti-Rb. It was concluded that there are specific interactions among Rb, HDAC1 and E2F1 proteins in gallbladder carcinoma, indicating the existence of the direct Rb/E2F1/HDAC1 signal transduction pathway. There is no direct relationship between p16 proteins with Rb, HDAC1, and E2F1 proteins.