Expression and Immunoreactivity of a Human Group A Rotavirus Vp4
- Author:
Qing-huan, ZHAO
;
Yu-ling, WEN
;
Yang, YU
;
Qing, DAI
;
Yuan-ding, CHEN
- Publication Type:Journal Article
- Keywords:
Recombinant rotavirus VP4;
Prokaryotic expression;
Immunoreactivity
- From:Virologica Sinica
2007;22(4):287-293
- CountryChina
- Language:Chinese
-
Abstract:
Rotavirus capsid protein Vp4 plays an important role in the virus adhering and entering the cells. In this study, a Vp4 gene cloned from a rotavirus strain TB-Chen was highly expressed in E.coli BL21 (DE3). The results of the Western blot showed that the protein possesses specific immuno-reactivities and can be specifically recognized by guinea pig antibodies against rotavirus strain SA11 or Wa. Some Vp4 dimers were formed during renaturation. These data obtained from this study provide a strong basis for further study on the structure and function of the Vp4.
