Identification and distribution of the clinical isolates of imipenem-resistant Pseudomonas aeruginosa carrying metallo-beta-lactamase and/or class 1 integron genes.
- Author:
Xi, CHENG
;
Pinjia, WANG
;
Yue, WANG
;
Hong, ZHANG
;
Chuanmin, TAO
;
Weiqing, YANG
;
Mei, LIU
;
Wenxiang, JIA
- Publication Type:Journal Article
- MeSH:
Anti-Bacterial Agents/pharmacology;
China;
DNA Primers/chemistry;
Drug Resistance, Bacterial;
Gene Expression Regulation, Bacterial;
Imipenem/*pharmacology;
Integrons;
Microbial Sensitivity Tests;
Models, Genetic;
Pseudomonas Infections/genetics;
Pseudomonas Infections/*microbiology;
Pseudomonas aeruginosa/*metabolism;
Sequence Analysis, DNA;
beta-Lactamases/*metabolism
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2008;28(3):235-8
- CountryChina
- Language:English
-
Abstract:
To investigate the distribution of the genes of two major metallo-beta-lactamases (MBL; i.e., IMP and VIM) and class 1 integrons (intI) in the clinical imipenem-resistant Pseudomonas aeruginosa, a total of 65 isolates, from a university hospital in Sichuan between December 2004 and April 2005 were screened for MBL genes by PCR using primers specific for bla ( IMP-1 ), bla ( VIM ) and bla ( VIM-2 ) genes. The MBL-positive isolates were further assessed for class 1 integrons by PCR using specific primers. The nucleotide sequences of several PCR products were also determined. The results revealed that the bla ( VIM ) gene was found in 81.5% (53/65) of all isolates, bla ( VIM-2 ) gene was found in only 1 isolate and the intI gene was observed in 45.3% (24/53) of bla ( VIM )-positive isolates. One isolate carried simultaneously both bla ( IMP-1 ) and intI genes, and to the best of our knowledge this is the first report of such isolate in southwest China. These observations highlight that the genes for VIM beta-lactamase and class 1 integrons were predominantly present among the imipenem-resistant P. aeruginosa tested, confirming the current widespread threat of imipenem-resistant, integron-borne P. aeruginosa.
