Differentiation of mesenchymal stem cells into nucleus pulposus cells in vitro.
- Author:
Fenghua, TAO
;
Feng, LI
;
Guanghui, LI
;
Feng, PAN
- Publication Type:Journal Article
- MeSH:
Aggrecans/metabolism;
Cell Differentiation;
Cells, Cultured;
Coculture Techniques;
Collagen/metabolism;
Gene Expression;
Gene Expression Regulation;
High Mobility Group Proteins/metabolism;
Intervertebral Disk/*cytology;
Mesenchymal Stem Cells/*cytology;
Mesenchymal Stem Cells/metabolism;
Models, Biological;
Reverse Transcriptase Polymerase Chain Reaction;
SOX9 Transcription Factor;
Tissue Engineering/instrumentation;
Tissue Engineering/*methods;
Transcription Factors/metabolism
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2008;28(2):156-8
- CountryChina
- Language:English
-
Abstract:
To find a new source of seed cells for constructing tissue-engineered intervertebral disc, nucleus pulposus (NP) cells and mesenchymal stem cells (MSCs) were isolated from New Zealand white rabbits. The nucleus pulposus cells population was fluorescence-laelled and co-cultured with MSCs with or without direct contact. Morphological changes were observed every 12 h. Semi-quantitative reverse transcriptase-polymerase chain reaction was performed to assess the expression levels of Sox-9, aggreacan and type II collagen every 24 h after the co-culture. MSCs treated with direct contact rounded up and presented a ring-like appearance. The expression of marker genes was significantly increased when cells were co-cultured with direct contact for 24 h. No significant change was found after coculture without direct contact. Co-culture of NP cells and MSCs with direct contact is a reliable method for generating large amount of NP cells used for cell-based tissue engineering therapy.
