beta-catenin/Tcf signaling in squamous differentiation of porcine airway epithelial cells.
- Author:
Wenshu, CHEN
;
Renliang, WU
;
Xi, WANG
- Publication Type:Journal Article
- MeSH:
Cell Differentiation;
Cell Nucleus/metabolism;
Cells, Cultured;
Cornified Envelope Proline-Rich Proteins;
Cytoplasm/metabolism;
Epithelial Cells/cytology;
Epithelial Cells/*metabolism;
Membrane Proteins/metabolism;
Mutation;
RNA, Messenger/metabolism;
Reverse Transcriptase Polymerase Chain Reaction;
Signal Transduction;
Swine;
Trachea/cytology;
Trachea/*metabolism;
Transcription, Genetic;
beta Catenin/*metabolism
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2008;28(2):121-4
- CountryChina
- Language:English
-
Abstract:
For a preliminary study of the role of beta-catenin/Tcf signaling in squamous differentiation of airway (tracheobronchial) epithelial cells, a stable mutant of beta-catenin was transfected into primarily cultured porcine airway epithelial cells. Western blotting revealed that exogenous protein was observed in large quantity in cytoplasm and nucleus. When co-transfected with Tcf luciferase reporter plasmids, beta-catenin mutant increased the reporter's transcriptional activities. However, mRNA expression of a squamous differentiation marker, small proline-rich protein (SPRP), was not elevated, as shown by reverse transcription-polymerase chain reaction. These findings suggest that beta-catenin/Tcf signaling may not be directly involved in the squamous differentiation of porcine airway epithelial cells.
