Lymphangiogenesis occurring in transplanted corneas.
- Author:
Shiqi, LING
;
Xiao, QING
;
Yanhua, HU
- Publication Type:Journal Article
- MeSH:
Cornea/*blood supply;
Cornea/chemistry;
Cornea/ultrastructure;
Corneal Neovascularization/etiology;
Corneal Neovascularization/metabolism;
Corneal Transplantation/adverse effects;
Corneal Transplantation/*methods;
Immunohistochemistry;
Lymphangiogenesis;
Microscopy, Electron;
Rats, Sprague-Dawley;
Rats, Wistar;
Vesicular Transport Proteins/biosynthesis
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2006;26(2):241-4
- CountryChina
- Language:English
-
Abstract:
To study corneal lymphangiogenesis after corneal transplantation, corneal allogenic transplantation models were established in rats. 8 female Wister rats were used as donors, and 16 Sprague Dawley (SD) rats were used as recipients and 2 SD served as controls. Corneal lymphangiogenesis and hemangiogenesis was examined by electron microscopy 1 and 2 weeks after corneal penetrating transplantation, and the expression of lymphatic vessel endothelial receptor (LYVE-1) was examined 1, 3, 7, 14 days after the transplantation respectively. In addition, 19 allograft failed human corneas were examined by 5'-nase-alkaline phosphatase (5'-NA-ALP) double-enzyme-histochemistry staining to detect corneal lymphangiogenesis and hemangiogenesis. By immunohistochemistry for LYVE-1, it was found that blown lymphatics were localized in the stroma 3 days after the corneal transplantation. With electron microscopy, new lymphatic vessels and blood vessels were found 1 and 2 weeks after the corneal transplantation. By 5'-NA-ALP enzyme-histochemistry, corneal hemangiogenesis was found in all allograft failed human corneas and 5 of 19 (26.3 %) cases had developed corneal lymphangiogenesis. It is concluded that corneal lymphangiogenesis is present after corneal transplantation, which may play an important role in allograft rejection.
