Angiogenic factor-enriched platelet-rich plasma enhances in vivo bone formation around alloplastic graft material.
10.4047/jap.2010.2.1.7
- Author:
Eun Seok KIM
;
Jae Jin KIM
;
Eun Jin PARK
- Publication Type:In Vitro ; Original Article
- Keywords:
Platelet-rich plasma;
Angiogenesis;
Human;
Athymic rat;
Cranial defect
- MeSH:
Angiogenesis Inducing Agents;
Animals;
Blood Platelets;
Bone Regeneration;
Calcium Phosphates;
Cell Proliferation;
Collagen;
Durapatite;
Humans;
Intercellular Signaling Peptides and Proteins;
Osteogenesis;
Perfusion;
Perfusion Imaging;
Platelet-Rich Plasma;
Rats, Nude;
Transplants;
Vascular Endothelial Growth Factor A
- From:The Journal of Advanced Prosthodontics
2010;2(1):7-13
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Although most researchers agree that platelet-rich plasma (PRP) is a good source of autogenous growth factors, its effect on bone regeneration is still controversial. The purpose of this study was to evaluate whether increasing angiogenic factors in the human PRP to enhance new bone formation through rapid angiogenesis. MATERIAL AND METHODS: In vitro, the human platelets were activated with application of shear stress, 20 microgram/ml collagen, 2 mM CaCl2 and 10U thrombin/1 x 109 platelets. Level of vascular endothelial growth factor (VEGF) and platelet microparticle (PMP) in the activated platelets were checked. In the animal study, human angiogenic factors-enriched PRP was tested in 28 athymic rat's cranial critical bone defects with beta-TCP. Angiogenesis and osteogenesis were evaluated by laser Doppler perfusion imaging, histology, dual energy X-ray densinometry, and micro-computed tomography. RESULTS: In vitro, this human angiogenic factors-enriched PRP resulted in better cellular proliferation and osteogenic differentiation. In vivo, increasing angiogenic potential of the PRP showed significantly higher blood perfusion around the defect and enhanced new bone formation around acellular bone graft material. CONCLUSION: Angiogenic factor-enriched PRP leads to faster and more extensive new bone formation in the critical size bone defect. The results implicate that rapid angiogenesis in the initial healing period by PRP could be supposed as a way to overcome short term effect of the rapid angiogenesis.
