Detection of Prevotella intermedia and Prevotella nigrescens using Pn17 and Pn34 DNA Probes.
- Author:
Chan Ho PARK
1
;
Pan Soon KIM
;
Hwa Sook KIM
;
Jeong Bum MIN
;
Ho Keel HWANG
;
Hyun Sun JANG
;
Ki Woon CHO
;
Dong Heon BAEK
;
Joong Ki KOOK
Author Information
1. Department of Conservative Dentistry, Chosun University 375 Seo-Suk Dong, Dong-ku, Gwang-ju 501-759, Korea.
- Publication Type:Original Article
- Keywords:
DNA probes Pn17 and Pn34;
Prevotella intermedia;
Prevotella nigrescens
- MeSH:
Blotting, Southern;
Chimera;
DNA;
DNA Probes;
DNA-Directed RNA Polymerases;
Polymorphism, Restriction Fragment Length;
Prevotella;
Prevotella intermedia;
Prevotella nigrescens;
Sensitivity and Specificity
- From:International Journal of Oral Biology
2010;35(1):13-19
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The DNA probes Pn17 and Pn34 were evaluated for their ability to specifically detect clinical strains of P. intermedia and P. nigrescens from a Korean population by dot blot hybridization. These probes were sequenced by extension termination and their specificity was determined by Southern blot analysis. The results revealed that the Pn17 sequence (2,517 bp) partially encodes an RNA polymerase beta subunit (rpoB) and that Pn34 (1,918 bp) partially encodes both rpoB (1-169 nts) and the RNA polymerase beta subunit (rpoB'; 695-1918 nts). These probes hybridized with both HindIII- and PstI-digested genomic DNAs from the strains of P. intermedia and P. nigrescens used in this study. Interestingly, each of the hybrid bands generated from the HindIII-digested genomic DNAs of the two bacterial species could be used to distinguish between them via restriction fragment length polymorphism. These results thus indicate that Pn17 and Pn34 can simultaneously detect P. intermedia and P. nigrescens.
