Development of Species-Specific PCR Primers for the Detection of Streptococcus sobrinus.
- Author:
Sang Gon KIM
1
;
So Young YOO
;
Joong Ki KOOK
Author Information
1. Department of Human Biology, School of Dentistry, Chosun University 375 Seo-Suk Dong, Dong-ku, Gwangju 501-759, Korea.
- Publication Type:Original Article
- Keywords:
detection;
PCR primers;
S. sobrinus
- MeSH:
Bacteria;
Clinical Coding;
Collodion;
DNA;
Polymerase Chain Reaction;
RNA, Ribosomal, 16S;
Sensitivity and Specificity;
Streptococcus;
Streptococcus sobrinus
- From:International Journal of Oral Biology
2010;35(1):21-25
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
This study was undertaken to develop species-specific forward and universal reverse PCR primers for the detection of Streptococcus sobrinus. These primers target the variable regions of the 16S ribosomal RNA coding gene (rDNA) and their specificity was tested against 10 strains of S. sobrinus strains and 20 different species of oral bacteria using serial dilutions of the purified genomic DNA of S.sobrinus ATCC 33478T . Our data show that species-specific amplicons were obtained from all the S. sobrinus strains tested but not from other species. Both direct and nested PCR could detect as little as 400 pg and 4 fg of genomic DNA from S. sobrinus ATCC 33478T , respectively. This result suggests that these PCR primers are highly specific and sensitive and applicable to the detection of S. sobrinus.
