Expression of telomerase subunits in gastric cancer.
- Author:
Fenghua, CHEN
;
Lihua, HU
;
Yirong, LI
;
Lin, WANG
- Publication Type:Journal Article
- MeSH:
Carrier Proteins/biosynthesis;
Carrier Proteins/genetics;
RNA/biosynthesis;
RNA/genetics;
RNA, Messenger/biosynthesis;
RNA, Messenger/genetics;
Reverse Transcriptase Polymerase Chain Reaction;
Stomach Neoplasms/*metabolism;
Telomerase/*biosynthesis;
Telomerase/genetics
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2005;25(6):741-3
- CountryChina
- Language:English
-
Abstract:
To detect the expression of telomerase subunits (human telomerase reverse transcriptase, human telomerase associated protein 1 and human telomerase RNA) in gastric cancer and to examine the role that different telomerase subunits play in the gastric carcinogenesis, reverse transcription-polymerase chain reaction (RT-PCR) was used to detect telomerase subunits messenger RNA in 24 samples of gastric cancer and corresponding non-cancerous tissue. The results showed that the positive rate of hTERT mRNA from gastric cancer and corresponding non-cancerous tissues was 100% and 25%, respectively. The former was significantly higher than the latter (chi2 = 26.4, P < 0.01). The positive rate of hTEP1 mRNA from gastric cancer and corresponding non-cancerous tissues was 100% and 91.7%, respectively and no significant difference was found between them (chi2 = 2.1, P > 0.05). The positive rates of hTR for gastric cancer and corresponding non-cancerous tissues were both 100% and no significant difference existed between them. It is concluded that in contrast to hTEP1 and hTR, the up-regulation of hTERT mRNA expression may play a more important role in the development of gastric cancer.
