Effects of 2-APB on store-operated Ca2+ channel currents of hepatocytes after hepatic ischemia/reperfusion injury in rats.
- Author:
Changzhou, HUANG
;
Zongming, ZHANG
;
Fazu, QIU
- Publication Type:Journal Article
- MeSH:
Boron Compounds/*pharmacology;
Calcium Channel Blockers/*pharmacology;
Calcium Channels/drug effects;
Cell Separation;
Hepatocytes/metabolism;
Liver/*blood supply;
Liver/metabolism;
Rats, Sprague-Dawley;
Reperfusion Injury/*metabolism
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2005;25(1):39-41
- CountryChina
- Language:English
-
Abstract:
The effects of hepatic ischemia/reperfusion (1/R) injuries on hepatocellular viability and store-operated calcium current (Isoc) in isolated rat hepatocytes and the effects of 2-APB on store-operated calcium current (Isoc) in isolated rat hepatocytes after hepatic ischemia/reperfusion injuries were studied. Hepatic ischemia and reperfusion injury model was established and whole cell patch-clamp techniques were used to investigate the effects of 2-APB on Isoc. The results showed that ischemia/reperfusion injuries could significantly reduce hepatocellular viability and further increase Isoc in hepatocytes and 2-APB (20, 40, 60, 80, 100 micromol/L,) produced a concentration-dependent decrease of Isoc with IC50 value of 64. 63 +/- 10.56 micromol/L, (n = 8). It was concluded that ischemia/reperfusion injuries could reduce hepatocellular viability, probably through increased Isoc in hepatocytes and 2-APB had a protective effect on ischemia/reperfusion-induced liver injury, probably though inhibiting Isoc.
