Transfection efficiency comparison of oligonucleotide and plasmid to the HL-60 cell line with liposomes.
- Author:
Yi, TANG
;
Wenli, LIU
;
Jianfeng, ZHOU
;
Huizhen, XU
;
Wu, LU
- Publication Type:Journal Article
- MeSH:
Green Fluorescent Proteins/genetics;
HL-60 Cells;
Liposomes;
Oligonucleotides/*genetics;
Plasmids/*genetics;
Transfection
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2005;25(1):24-5
- CountryChina
- Language:English
-
Abstract:
The transfection efficiency of oligonucleotide and plasmid to the HL-60 cell line with lipofectaminePLUS was compared through observing the transfection rate and the expression duration of exogenous gene in the target cells. The results showed that the transfection rate of oligonucleotide to the HL-60 was about 90% - 95% and it had no obvious attenuation within 84 h. However, the plasmid transfection rate was only 5% -25% and it was decreased significantly within 60 h. It was suggested that the transfection of oligonucleotide with liposomes was better than that of plasmid.
