KLF6mRNA expression in primary hepatocellular carcinoma.
- Author:
Shaoping, WANG
;
Xiaoping, CHEN
;
Wanguang, ZHANG
;
Fazu, QIU
- Publication Type:Journal Article
- MeSH:
Carcinoma, Hepatocellular/*metabolism;
Kruppel-Like Transcription Factors/*biosynthesis;
Kruppel-Like Transcription Factors/genetics;
Liver/metabolism;
Liver Neoplasms/*metabolism;
Proto-Oncogene Proteins/*biosynthesis;
Proto-Oncogene Proteins/genetics;
RNA, Messenger/biosynthesis;
RNA, Messenger/genetics;
Reverse Transcriptase Polymerase Chain Reaction
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2004;24(6):585-7
- CountryChina
- Language:English
-
Abstract:
To investigate the expression of KLF6mRNA in primary hepatocellular carcinoma (HCC), nomal liver tissues and the tissues adjacent to the cancers, reverse-transcription polymerase chain reaction (RT-PCR) was employed to investigate the expression of the KLF6 gene in HCC, the corresponding adjacent non-cancerous tissues and normal liver tissue. Our results showed that an amplified fragment of 427 bp DNA was detected in 18 of 19 (94.7%) adjacent non-cancerous tissues and normal liver tissue, and in 12 (85.7%) of 14 HCC. There were no significant differences in the levels of KLF6 mRNA between normal liver and liver tumors (P>0.05). It is concluded that KLF6 mRNA is generally expressed in HCC.
