Expression of transforming growth factor-beta in cultured normal human lens epithelia cells.
- Author:
Yukan, HUANG
;
Houren, WEI
- Publication Type:Journal Article
- MeSH:
Cells, Cultured;
Epithelial Cells/cytology;
Epithelial Cells/*metabolism;
Lens, Crystalline/cytology;
Lens, Crystalline/*metabolism;
RNA, Messenger/biosynthesis;
RNA, Messenger/genetics;
Transforming Growth Factor beta/*biosynthesis;
Transforming Growth Factor beta/genetics
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2004;24(3):289-91
- CountryChina
- Language:English
-
Abstract:
In order to investigate whether cultured normal human lens epithelial cells (LEC) express transforming growth factor beta (TGF-beta), reverse transcriptase polymerase chain reaction (RT-PCR) and immunohistochemical methods were used for detection of TGF-beta mRNA and protein in cultured normal human LEC. The results showed that a single RT-PCR amplified product about 310bp was obtained, and the sequence was homologous to the known sequence. TGF-beta immunostain was positive in the plasma of LEC. It was suggested that normal human LEC could produce TGF-beta, and LEC could be affected by TGF-beta through autocrine action.
