Protective effect of GSH on PD model induced by 6-OHDA in vitro.
- Author:
Yan, XU
;
Shenggang, SUN
;
Xuebing, CAO
;
Etang, TONG
- Publication Type:Journal Article
- MeSH:
Glutathione/*therapeutic use;
Neurons/pathology;
Oxidopamine;
Parkinson Disease, Secondary/chemically induced;
Parkinson Disease, Secondary/*drug therapy;
Random Allocation;
Rats, Sprague-Dawley;
Substantia Nigra/pathology;
Tyrosine 3-Monooxygenase/metabolism
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2002;22(4):355-8
- CountryChina
- Language:English
-
Abstract:
To study the effects of 6-hydroxydopamine (6-OHDA) and reduced glutathione (GSH) on the nigral dopaminergic neurons in brain slices in vitro, immolunohistochemical technique was used to observe the changes of TH-stained neurons, including cell bodies and the dendrites, in the substantia nigra (SN) of midbrain slices of rats after incubation for 1 h in the presence of GSH 15 min before and during the period of incubation with 6-OHDA. The results showed that cell bodies remained intact but dendrites were fragmented and truncated after treatment with 6-OHDA. The antioxidant GSH alone did not significantly affect the dendrites of SN neurons but prevented 6-O-HDA-induced damage of dendrites. It was concluded that glutathione may prevent 6-OHDA-induced dopaminergic neurodegeneration and play a protective role in dopaminergic neurons.
