Establishment and comparison of DHPG and BMI induced epileptic discharge models
- VernacularTitle:DHPG和BMI诱导的癫痫样放电模型的建立及比较
- Author:
Li, JING
;
Qin-chi, LU
- Publication Type:Journal Article
- Keywords:
dihydroxyphenylglycine;
bicuculline methiodide;
hippocampus;
oscillation;
epileptic discharge
- From:Journal of Shanghai Jiaotong University(Medical Science)
2009;29(6):668-672
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop models of epileptic discharge by activating group Ⅰ metabotropic glutamate receptors (mGluR) or by blocking gamma-aminobutyric acid (GABA) receptors on rat hippocampal slices. Methods Rat hippocampal slices were exposed to mGluR group Ⅰ specific agonist dihydroxyphenylglycine (DHPG) or to GABAA receptor antagonists bicuculline methiodide(BMI), and single pyramidal cell in the CA3 region of the slice was recorded by whole cell patch clamp technique. Results Exposure to DHPG or BMI resulted in the induction of spontaneously occurring epileptic discharge in the CA3 region of rat hippocampal slice, and there was no significant difference in the frequency of discharge between them(P>0.05). Conclusion Epileptic discharge can be generated in vitro in response to a loss of balance between excitatory and inhibitory influences.
