An experimental study on the expression of heme oxygenase-2 mRNA in Hirschsprung's disease.
- Author:
Min, ZHU
;
Mingfa, WEI
;
Fang, LIU
- Publication Type:Journal Article
- MeSH:
Colon/enzymology;
Heme Oxygenase (Decyclizing)/biosynthesis;
Heme Oxygenase (Decyclizing)/*genetics;
Hirschsprung Disease/*enzymology;
Hirschsprung Disease/etiology;
Hirschsprung Disease/genetics;
RNA, Messenger/biosynthesis;
RNA, Messenger/genetics
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2002;22(2):152-4
- CountryChina
- Language:English
-
Abstract:
In order to investigate the relationship between the expression of heme oxygenase-2 (HO-2) mRNA and the pathogenesis of Hirschsprung's disease (HD), total ribonucleic acid (RNA) was extracted in the aganglionic and ganglionic segments of colon respectively from 15 cases of HD. The single-stranded cDNA of HO-2 was synthesized and further amplified by reverse transcription-polymerase chain reaction (RT-PCR). The expression of HO-2 mRNA was normal in ganglionic segments, but absent in aganglionic segments. It is concluded that the absence of HO-2 mRNA expression may be an important mechanism responsible for HD.
