Construction and expression of recombinant plasmid pCD-rbFGF in osteoblasts.
- Author:
Cao, YANG
;
Shuhua, YANG
;
Xiaodong, GUO
;
Shen, QU
- Publication Type:Journal Article
- MeSH:
Cells, Cultured;
DNA, Complementary/genetics;
Escherichia coli/genetics;
Eukaryotic Cells/metabolism;
Fibroblast Growth Factor 2/biosynthesis;
Fibroblast Growth Factor 2/*genetics;
Gene Transfer Techniques;
Osteoblasts/cytology;
Osteoblasts/*metabolism;
Plasmids;
Recombinant Proteins/biosynthesis;
Recombinant Proteins/genetics;
*Transfection;
Transformation, Genetic
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2002;22(2):109-11
- CountryChina
- Language:English
-
Abstract:
To construct basic fibroblast growth factor (bFGF) eukaryotic expression vector and to evaluate the possibility of bFGF gene therapy in orthopedic disease, the pCD-rbFGF recombinant plasmid was constructed by cloning rat basic fibroblast growth factor (bFGF) cDNA into an eukaryotic expression vector, pcDNA3. Rat osteoblasts were transfected with pCD-rbFGF plasmid by lopofectin mediated gene transfer, the transient expression was detected by streptavidin-biotin-enzyme complex (SABC) method. It was observed that the expression of rat bFGF gene was detected 72 h after transfected distinctly. Basic fibroblast growth factor gene therapy is a method of potential for a wide array of orthopedic diseases.
