Modulation of transglutaminase expression in rat skeletal muscle by induction of atrophy and endurance training.
10.3346/jkms.1994.9.6.490
- Author:
Sang Chul PARK
1
;
Woo Ho KIM
;
Myung Chul LEE
;
Sang Cheol SEONG
;
Kye Yong SONG
;
Myoung Ae CHOE
Author Information
1. Department of Biochemistry, WHO Collaborating Centre on Physical Culture and Aging Research for Health Promotion, Seoul, Korea.
- Publication Type:Original Article ; Comparative Study ; Research Support, Non-U.S. Gov't
- Keywords:
Muscle atrophy;
Transglutaminases;
Exercise
- MeSH:
Animal;
Atrophy;
Comparative Study;
Enzyme Induction;
Female;
Hindlimb;
Immobilization;
Muscle Fibers/pathology;
Muscle Proteins/*biosynthesis;
Muscles/*enzymology/pathology;
Physical Conditioning, Animal;
*Physical Endurance;
Rats;
Rats, Wistar;
Support, Non-U.S. Gov't;
Swimming;
Transglutaminases/*biosynthesis
- From:Journal of Korean Medical Science
1994;9(6):490-496
- CountryRepublic of Korea
- Language:English
-
Abstract:
The persistence of muscle fiber number regardless of size reduction in muscle atrophy has not yet been fully explained. For the mechanism inherent in skeletal muscle tissues for preventing cellular death, the protective function of muscle tissue through transglutaminases has been tested, since the enzyme is responsible for structural stabilization and participates in signal transduction. In the present experiment, hindlimb suspension for two weeks caused a marked muscle atrophy in Wistar female rats. Comparison of muscle weight and histological analysis showed that suspension-induced atrophy in the hindlimb was more prominent in the soleus muscle, comprised mainly of type I fiber than that in the plantaris muscle of type II fibers. The immunohistochemical analysis with antitransglutaminase C antibody (anti TGase C Ab) showed that some atrophic bundles of soleus muscle were positively reacted with the antibody. The anti-TGase C Ab-reactive substances were observed to disappear significantly after endurance exercise, indicating their characteristic atrophy-dependency. The enzymatic analysis of transglutaminase showed the increase in activity in the atrophic soleus muscle tissue, compared with that in the normal or exercise-trained muscle tissues. From these results, the expression of TGase in the atrophic muscle is suggested to be the possible marker for muscle atrophy and its expression is probably related with the protective mechanism of the muscle tissue to prevent further cellular damage in the atrophic process.
