Comparison of the in-vitro effects of bevacizumab, mitomycin-C, 5-fluorouracil,and triamcinolone acetonide on the viability of cultured human tenon's fibroblasts.
- Author:
Pius Jonas Ocampo
1
;
Ma. Margarita Lat-Luna
1
,
2
;
Joseph Anthony Tumbocon
1
,
3
;
Polly Chao-Po
1
;
Andrei Martin
1
Author Information
- Publication Type:Journal Article
- MeSH: Human; Male; Female; .humans; Mitomycin; Triamcinolone Acetonide; Trabeculectomy; Resazurin; Bevacizumab; Fluorouracil; Cell Survival; Control Groups; Inhibitory Concentration 50; Tenon Capsule; Xanthenes; Oxazines; Antibodies, Monoclonal, Humanized; Fibroblasts; Software
- From: Philippine Journal of Ophthalmology 2014;39(2):84-89
- CountryPhilippines
- Language:English
-
Abstract:
Objective: To evaluate and compare the effects of bevacizumab, mitoinycin-C (MMC), 5-fluorouracil (5-FU), and triamcinolone acetonide (TA) on the viability of cultured human Tenon's capsule fibroblasts (cHTF) in vitro.
Methods: Human Tenon's fibroblasts (HTF) were harvested and cultured in a Roswell-Park-Memorial 1-Institute (RPMI) media. MMC, 5-FU, bevaciz. umab, and TA were administered to the cHTF at 3-fold decreasing concentrations starting from 20 ug, 5 mg, 25 mg, and 4 mg respectively. A negative control/untreated group containing RPMI media only was included in the study. Fibroblast cell viability was assessed using resazurin fluorim etric assay. Half¬maximal inhibitory concentration (IC50) was computed for agents which showed significant decrease in cHTF viability compared to the untreated group.
Results: There was no significant difference in cH IF viability between the untreated control group compared to 5-FU (p=0.97), bevacizumab (p=0.10), and TA (p=0.06) groups. Mitomycin-C showed a significant decrease in cHTF viability (p<0.001) which was dose dependent. The IC50 of MMC was computed at 12.16 ug using the prism software.
Conclusion: Mitomycin-C demonstrated dose-dependent decrease in viability of cultured human Tenon's fibroblasts. 5-FU, bevacizumab, and triamcinolone did not show this effect.
Key Words: Mitomycin-C, 5-fluorouracil, Bevaciz. umab, Tria. mcinolone acetonide, Fibroblast, Trabeculectomy
- Full text:PJO 49.pdf