Eosinophil Inflammation of Nasal Polyp Tissue: Relationships with Matrix Metalloproteinases, Tissue Inhibitor of Metalloproteinase-1, and Transforming Growth Factor-beta1.
10.3346/jkms.2003.18.1.97
- Author:
Hae Sim PARK
1
;
Sun Sin KIM
;
Hyun Ah KIM
;
Yu Jin SUH
;
Soo Keol LEE
;
Dong Ho NAHM
;
Young Mok LEE
Author Information
1. Department of Allergy and Clinical Immunology, Ajou University School of Medicine, Suwon, Korea. hspark@madang.ajou.ac.kr
- Publication Type:Original Article
- Keywords:
Nasal Polyps;
Matrix Metalloproteinases;
Tissue-Inhibitor of Metalloproteinase-1;
Transforming Growth Factor beta;
Eosinophils
- MeSH:
Adult;
Asthma/complications;
Blood Proteins/analysis;
Chemotaxis, Leukocyte;
Eosinophilia/etiology;
Eosinophilia/metabolism*;
Eosinophilia/pathology;
Eosinophils/physiology;
Female;
Gelatinase A/analysis;
Gelatinase A/physiology*;
Gelatinase B/analysis;
Gelatinase B/physiology*;
Human;
Male;
Mast Cells/physiology;
Middle Aged;
Nasal Polyps/chemistry*;
Nasal Polyps/etiology;
Nasal Polyps/pathology;
Rhinitis/metabolism;
Rhinitis/pathology;
Ribonucleases*;
Serine Endopeptidases/analysis;
Tissue-Inhibitor of Metalloproteinase-1/analysis;
Tissue-Inhibitor of Metalloproteinase-1/physiology*;
Transforming Growth Factor beta/analysis;
Transforming Growth Factor beta/physiology*
- From:Journal of Korean Medical Science
2003;18(1):97-102
- CountryRepublic of Korea
- Language:English
-
Abstract:
Eosinophil and mast cell infiltrations are consistent findings in nasal polyp tissue. Previous studies have shown that matrix metalloproteinases (MMPs) may be involved in eosinophil infiltration in airway mucosa of asthmatic patients, and that transforming growth factor-beta1 (TGF-beta1) induces extracellular matrix deposition in nasal polyp tissue. The aim of this study was to evaluate the role of MMPs and tissue-inhibitor of metalloproteinase-1 (TIMP-1) in association with TGF-beta1, eosinophils and mast cell activation in nasal polyp tissue. Nasal polyp tissues from 20 patients who underwent polypectomies were collected and prepared into tissue homogenate. Eosinophil cationic protein (ECP) and tryptase levels were measured by CAP system (Pharmacia, Sweden). MMP-2, MMP-9, TIMP-1 and TGF-beta1 levels were measured by enzyme-liked immunosorbent assay. MMP-2 was the predominant form of MMPs, followed by MMP-9 and TIMP-1. There were significant correlations between ECP, and MMP-9, MMP-2, TGF-beta1 and tryptase, but not with TIMP-1. Significant correlations were noted between tryptase, and MMP-2, MMP-9, and TGF-beta1, but not with TIMP-1. Close correlations were noted between TGF-beta1, and MMP-9 and MMP-2, but not with TIMP-1. MMP-2, MMP-9, and TGF-beta1 may contribute to eosinophil and mast cell migrations into nasal polyp tissue.
