Distribution of Adenoviral Vector in Brain after Intravenous Administration.
10.3346/jkms.2003.18.1.108
- Author:
Jong Youl JIN
1
;
Chan Il MOON
;
Che Il MOON
;
Wha Sun KANG
;
Dae Chul JEONG
Author Information
1. Department of Internal Medicine, Holy Family Hospital, College of Medicine, The Catholic University of Korea, Bucheon, Korea. jyjin@hfh.cuk.ac.kr
- Publication Type:Original Article ; Comparative Study
- Keywords:
Adenoviridae;
Central Nervous System;
Green Fluorescent Protein;
Gene Therapy
- MeSH:
Adenoviruses, Human/isolation & purification*;
Animals;
Blood-Brain Barrier;
Brain/virology*;
Cerebellum/cytology;
Cerebellum/virology;
Comparative Study;
Female;
Genes, Reporter;
Genetic Vectors/administration & dosage;
Genetic Vectors/isolation & purification;
Genetic Vectors/pharmacokinetics*;
Hippocampus/virology;
Inferior Colliculus/virology;
Injections, Intravenous;
Luminescent Proteins/analysis;
Luminescent Proteins/biosynthesis;
Luminescent Proteins/genetics;
Mice;
Mice, Inbred BALB C;
Neuroglia/virology;
Neurons/virology*;
Purkinje Cells/virology;
Pyramidal Cells/virology;
Recombinant Fusion Proteins/analysis;
Recombinant Fusion Proteins/biosynthesis;
Recombinant Fusion Proteins/genetics;
Tail/blood supply;
Tissue Distribution
- From:Journal of Korean Medical Science
2003;18(1):108-111
- CountryRepublic of Korea
- Language:English
-
Abstract:
The delivery of transgenes to the central nervous system (CNS) can be a valuable tool to treat CNS diseases. Various systems for the delivery to the CNS have been developed; vascular delivery of viral vectors being most recent. Here, we investigated gene transfer to the CNS by intravenous injection of recombinant adenoviral vectors, containing green fluorescence protein (GFP) as a reporter gene. Expression of GFP was first observed 6 days after the gene transfer, peaked at 14 days, and almost diminished after 28 days. The observed expression of GFP in the CNS was highly localized to hippocampal CA regions of cerebral neocortex, inferior colliculus of midbrain, and granular cell and Purkinje cell layers of cerebellum. It is concluded that intravenous delivery of adenoviral vectors can be used for gene delivery to the CNS, and hence the technique could be beneficial to gene therapy.
