Anti-interleukin-33 Reduces Ovalbumin-Induced Nephrotoxicity and Expression of Kidney Injury Molecule-1.
- Author:
Geun Ho PARK
1
;
Helen Ki SHINN
;
Ju Hee KANG
;
Won Ju NA
;
Young Hyo KIM
;
Chang Shin PARK
Author Information
- Publication Type:Original Article
- Keywords: Acute Kidney Injury; Interleukin-33; Ovalbumin; Kidney Injury Molecule-1
- MeSH: Acute Kidney Injury; Adenylate Kinase; Animals; Blood Urea Nitrogen; Creatinine; Cyclooxygenase 2; Down-Regulation; Female; Humans; Immunoglobulin G; Interleukin-33; Kidney*; Mice; Nitric Oxide Synthase Type II; Ovalbumin; Ovum; RNA, Messenger
- From:International Neurourology Journal 2016;20(2):114-121
- CountryRepublic of Korea
- Language:English
- Abstract: PURPOSE: To evaluate the effect of anti-interleukin-33 (anti-IL-33) on a mouse model of ovalbumin (OVA)-induced acute kidney injury (AKI). METHODS: Twenty-four female BALB/c mice were assigned to 4 groups: group A (control, n=6) was administered sterile saline intraperitoneally (i.p.) and intranasally (i.n.); group B (allergic, n=6) was administered i.p./i.n. OVA challenge; group C (null treatment, n=6) was administered control IgG i.p. before OVA challenge; and group D (anti-IL-33, n=6) was pretreated with 3.6 µg of anti-IL-33 i.p. before every OVA challenge. The following were evaluated after sacrifice: serum blood urea nitrogen and creatinine levels, Kidney injury molecule-1 gene (Kim-1) and protein (KIM-1) expression in renal parenchyma, and expression of cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), phosphorylated endothelial NOS (p-eNOS), and phosphorylated AMP kinase (p-AMPK) proteins in renal parenchyma. RESULTS: After OVA injection and intranasal challenge, mice in groups B and C showed significant increases in the expression of Kim-1 at both the mRNA and protein levels. After anti-IL-33 treatment, mice in group D showed significant Kim-1 down-regulation at the mRNA and protein levels. Group D also showed significantly lower COX-2 protein expression, marginally lesser iNOS expression than groups B and C, and p-eNOS and p-AMPK expression at baseline levels. CONCLUSIONS: Kim-1 could be a useful marker for detecting early-stage renal injury in mouse models of OVA-induced AKI. Further, anti-IL-33 might have beneficial effects on these mouse models.
