SPECTROPHOTOMETRIC ANALYSIS OF THE ETHANOL EXTRACTS FROM THE RHODODENDRON ADAMSII REHD
- VernacularTitle:АДАМСЫН ТЭРЭЛЖ (RHODODENDRON ADAMSII REHD)-НИЙ СПИРТЭН ХАНДНЫ СПЕКТРОФОТОМЕТРИЙН ШИНЖИЛГЭЭ
- Author:
Х.Золжаргал
;
Г.Ганчимэг
;
Э. Наранчимэг
;
Б.Цэрэндолгор
- Publication Type:Journal article
- Keywords:
Rhododendron Adamsii Rehd, total flavonoid, total carotenoid
- From:
Mongolian Pharmacy and Pharmacology
2017;11(2):22-26
- CountryMongolia
- Language:Mongolian
-
Abstract:
Introduction: In recent years, due to the increase in population density, noisiness and
air pollution, people’s life rhythm has been changing dramatically and it is creating stress
both mentally and physically for human beings. Therefore, this research is to extract a
pill from an adaptogenic domestic herb Rhododendron Adamsi Rehd using its parts on the
earth surface.
Materials and methods: Analysis of determination biologically active substances in the
ethanol extracts from the Rhododendron Adamsii Rehd. The main material for the research
is the aerial parts of Rhododendron Adamsi Rehd which grows on the earth surface during
the blooming period of the herb in the area of Ulaan-Uul soum in Khuvsgul province. Also
following materials were used in the research: Spectrophotometer (UV/VIS 1700), electric
measurement, soxhlet extractor, ethanol, aluminum chloride, Sodium nitrite, sodium
hydroxide solution. Rhododendron Adamsii Rehd extracting in 40% and 70% ethyl alcohol
60, 120 and 180 minutes (reflux) are extracted and spectrometric survey found the total
flavonoid content, the most sensitive to wavelengths that extra cts from each method.
Results: Rhododendron Adamsii Rehd are proven to be 60 minutes for 40% of ethanol
extraction is suitable for the determination of total flavonoids. We are researching of this
extract most sensitive wavelength between 190-500 nm. This research result shown that
total flavonoid 0,648-0,67 light absorption at a wavelength of 255-257 nm; 258-259 nm
wavelength light absorption 0,673; 260-262 nm were 0,66-0,67 light absorption. The fact
proven to be most sensitive wavelength of 40% ethanol extract is 258-259 nm. Rhododendron
Adamsii Rehd were suitable for extraction of 70% ethanol during 60-120 minutes. Total
flavonoid of this extract absorption 1,478-1,757 at a wavelength 250-254 nm 255-256 nm
wavelength light absorption 1,764-1,788; 1,731 at a wavelength 260 nm light absorption.
This shows that proven to be most sensitive to wavelength of 70% ethanol extract is 255-
256 nm. Rhododendron Adamsii Rehd water extraction agent 30 ± 0.01% (p <0.001) and
total flavonoids 40% of ethanol extract content: 1.77-2,6 ± 0.03% (p <0.0001); 70% of
ethanol extract content: 2,4-7.46 ± 0.01% (p <0.0001).
Сonclusion: The study results were total carotenoid 0.278 ± 0.03% of 40% ethanol
extract (p <0.005) and total carotenoid 1,263± 0.05% (p <0.001) of 70% ethanol extract.
Key words: Rhododendron Adamsii Rehd, total flavonoid, total carotenoid
