Development and Validation of an UV Spectrophotometric Method of Quantification of Fluconazole Reference
- VernacularTitle:Стандарт флуконазолыг хят- ны спектрофотометрийн аргаар тодорхойлж баталгаажуулах нь
- Author:
Gereltuya Dorj
;
Sarnaizul Erdenebaatar
;
Zoljargal Sambuu
;
Nomin-Erdene Tsolmon
;
School of Pharmacy and Biomedicine, MNUMS, United family, Intermed hospital,National second hospita
- Publication Type:Journal article
- Keywords:
Fluconazole;
method development;
validation;
spectrophotometry
- From:
Mongolian Pharmacy and Pharmacology
2016;9(2):11-15
- CountryMongolia
- Language:Mongolian
-
Abstract:
Introduction: A UV-spectrophotometric method has been developed for the quantitative estimation
of fluconazole. The present study describes development and validation of UV-spectroscopic
method for estimation of fluconazole reference substance and validation of detection method.
Method: During development of analytical method HCl: water (6 different concentrations).
The detection length (cmax) was found to be 260nm. Calibration curves were prepared. The proposed
method obeys Beer’s law in the range of 5-50mkg/ml. Absorption maxima was determined
with 10 mkg/ml by scanning in the range of 200-400nm.
Results: Percent recovery studies are in the range of 95,12%-101.89%. The method was validated
in terms of specificity, linearity, accuracy, range, precision, repeatability, robustness, stability
of analytical solution. Results of analysis were validated statistically and by specificity studies.
From that it was observed that there is no interference of blank, excipients during the estimation
of reference substance in formulation.
Conclusion: This shows the adaptability of the method for routine estimation of fluconazole
reference. Keywords: Fluconazole, method development, validation, spectrophotometry
- Full text:W020170427507130091663.pdf
