ErbB4 Regulates the Intracellular Distribution of Surfactant Proteins in Alveolar Lpithelial Type II Cells
- VernacularTitle:ErbB4 Regulates the Intracellular Distribution of Surfactant Proteins in Alveolar Lpithelial Type II Cells
- Author:
ERDENEBULGAN Purevdorj
- Publication Type:journal article
- From:Innovation
2008;5(1):27-27
- CountryMongolia
- Language:English
-
Abstract:
Alveolar epithelial type II cclls (AE II) synthesize, store, and recycle surfactant. Lamellar bodies (lb) are the storage organelles of hydrophobic surfactant proteins (SP) and multivesicular bodies (mvb) of hydrophilic (SP-A and SP-D) and also the precursors of hydrophobic SP. ErbB4 is an important regulator of lung development and its deletion leads to an alveolar simplification, abnormal lung function, SP-D downrcgulation, and signs of chronical inflammation.
The objective of the study is to evaluate the effects of ErbB4 deletion on intracellular localization of SP and the structure of lb.
Volume fraction and size of lb was determined stcreologically in sections of ErbB4 deleted transgenic adult lungs (HER4hcan). Intracellular SP was detected by quantitative immunoelcctron microscopy and the relative labeling index (RLI).
Results: ErbB4 deletion had no significant effect on size of AE II and volume fractions of subcellular organelles. SP-C was predominantly distributed in lb and mvb independent of the genotype. SP-A was preferentially localized to the cytoplasm in the control and to the lb in the ErbB4 deleted AEII. ErbB4 deletion let to a change in SP-B localization from lb in controls to the mvb in the homozygote negative lung.
ErbB4 deletion docs not affect the structure of the sub cellular organelles in AE II. However, it interferes with the intracellular localization of SP-A and SP-B. The biologic relevance of these findings has to be elucidated. But it might be speculated that it has an effect on surfactant and overall lung function in these animals or is related to the chronic inflammation seen in these lungs.
ATS 2008 • Toronto International Conference
Filename: 955427
Abstract Category: 03.03 • PFI) - Developmental Lung Biology
