polysaccharide Treatment Potentiates the Structural Delay of f,ung Development in ErbB4-Deleted Fetal Lungs
- VernacularTitle:polysaccharide Treatment Potentiates the Structural Delay of f,ung Development in ErbB4-Deleted Fetal Lungs
- Author:
ERDENEBULGAN Purevdorj
- Publication Type:journal article
- From:Innovation
2008;5(1):27-27
- CountryMongolia
- Language:English
-
Abstract:
ErbB4 plays an important role in the developing lung. Deletion of ErbB4 delays structural development and the onset of fetal surfactant synthesis. Adult animals develop a hyper reactive airway system and alveolar simplification, similar to preterm infants with bronchopulmonary dysplasia. Antenatal lipopolysaccharide (LPS) exposure also interrupts structural development.
The objective .ot the study is to study me additive effects of antenatal LPS administration and ErbB4 deictic»"1 0,1 structural lung development and the resulting ErbB receptor regulation.
Pregnant HER4hca" mice were treated with LPS (100 g/kg, intra-pcritoncal) at d 17 of gestation. Lungs were isolated 24 hour later for stereological and ErbB receptor analyses.
LPS exposure resulted in delay of structural development leading to an increased volume density of lung mesenchyme and decreased volume density of ductal airspace. LPS-treatcd lungs were at the canalicular stage and saline-treated control lungs were at the saccular stage. ErbB4-deleted lungs treated with LPS showed an even further delay in structural lung development with the volume density of lung mesenchyme being increased by 70% and the volume density of ductal airspace decreased by 13% when compared to the ErbB4-deleted control lungs. Western blotting showed decreased phosphorylation, but increased ErbB2 protein expression in LPS-treatcd lungs.
ErbB4 deletion potentiates the LPS-induced delay in structural development implying a role of ErbB4 in response to inflammatory lung injury. ErbB2 is up-regulated in inflammation, implying a role in the signaling of this injury. It might be speculated that ErbB4 has a more protective and ErbB2 a more injury promoting role in inflammation.
A TS 2008 • Toronto International
Conference Filename: 952117
Abstract Category: 03.03 - PED - Developmental Lung Biology
