Study of snake venom derived anti-platelets aggregation
- VernacularTitle:Могойн хорноос гаралтай цусны ялтасын агрегацийг саатуулагч уургуудыг судалсан дүн
- Author:
Altantsetseg.B
;
Bat-Erdene.J
;
Punsaldulam.D
;
Bolor.B
;
Sumiya.B
;
Krasnobryzhaya. Ye. N
;
Volkov. G.L
;
Tseren. B
;
Tsogtkhishig.Ts
- Publication Type:journal article
- Keywords:
platelets aggregation,liquid chromatography,disintegrin,phospholipase
- From:Innovation
2014;8(1):54-60
- CountryMongolia
- Language:Mongolian
-
Abstract:
Snake venom toxins have been reportedly used as a rich source of a number of proteins
of biotechnological interest due to their wide range of effects on haemostasis. These
effects vary greatly: coagulant, anticoagulant, platelet-activating, anti-platelet, fibrinolytic
and hemorrhagic, in either enzymatic or non-enzymatic pathways. Agkistrodon venom
contains a variety of proteins that possess antiplatelet activities. This study presents
recent development in our laboratory to produce and purify antiplatelet proteins derived
from Agkistrodon blomhoffi ussuriensiss nake venom. Different matrices of HPLC (size
exclusion, ion exchange and affinity chromatography) were employed for purifying the
proteins and their biological and biochemical properties were characterized by SDSPAGE,
2-D electrophoresis, platelet aggregation assay and enzyme activity assay.A purified
disintegrin was a single chain glycoprotein with Mr of 13 kDа and рІ 4.7, whereas PLA2
had Mr of 14 kDa and pI of 4.17. A dose-dependent activity curve analysis shows that
the platelet aggregation inhibitory activity of disintegrin and PLA2 in the rabbit plateletrich
plasma were ID50 of 0.25 μМ and 0.65 μМ, respectively. Bioprocesses to produce
and purify active antiplatelet agents from A. blomhoffi ussuriensis venom have been
developed, using modern liquid chromatography matrices. Ongoing work to optimize
large-scale production process is being undertaken.
