The study of apolipoprotein a5 gene polymorphisms in relation tolipidslevel in people with metabolic syndrome
- VernacularTitle:Бодисын солилцооны хам шинжтэй хүмүүст аполипопротейн а5 генийн зарим хэв шинжүүдийг өөх тосны үзүүлэлтүүдтэй холбон судалсан дүн
- Author:
Ariunbold Ch
;
Buyankhuu T
;
Munkhtsetseg J
- Publication Type:Journal Article
- Keywords:
Metabolic Syndrome;
Cholesterol;
Triglyceride;
HDL;
ApoA5 gene;
SNPs
- From:Mongolian Medical Sciences
2014;170(4):10-13
- CountryMongolia
- Language:Mongolian
-
Abstract:
Background: Epidemiologic studies have shown a higher prevalenceof hypertriglyceridemia among
patients with CHDthan among unaffected populations. Dozens of polymorphisms in different genesthat
could have some effect on plasma TG levels havebeen analyzed.
The most promising results are connected withvariants within the apolipoproteins (APO) APOA1/APOC3/
APOA4 gene cluster. Transgenic mice overexpressing human apolipoprotein A5decreased plasma
triglyceride concentrations to one-third of those in control mice; conversely, knockout mice lacking
APOA5 had four times as much plasma triglycerides as controls.The human APOA5 gene consistsof
4 exons and codes 369 aminoacidprotein, which is expressed almost exclusively in the liver.A minor
allele of APOA5 (1259C, IVS3+476A and 1131C) which was independently associated with high plasma
triglyceride levels in African-American, non Hispanic whites, Hispanic, Caucasians and Japanese were
reported. Four polymorphisms in ApoA5 (1259T>C, IVS3+476G>A, S19W and 1131T>C) has been
correlatedwith high TG levels in diabetic women.
Materials and Methods: 162 people with MS for case group and 144 people for control group were
selected in this study. MS was diagnosed according to IDF criteria and serum triglyceride, total cholesterol
and HDL levels were determined. DNA from both case and control subjects were extracted from blood
samples (20μL) using “G-spin™ Total DNA Extraction Kit”(iNtRON Biotechnology, Inc).The genotypes
for fourpolymorphisms of ApoA5 were determined using a combination of PCR and sequence-specific
oligonucleotide probes.
Results: There were 304 total subjects included males 50.3% (153) and female 49.7% (151) in our study.
The appearance of risk genotypes of 1177C>T, 1259T>C, IVS3+476G>A and 1131T>C polymorphisms
in ApoA5 gene were higher in MS group than control group.Serum levels of triglycerides and total
cholesterol differed significantly (p<0.001, p=0.029) among APOA5-1131T>C genotypes.
Conclusion: TAG and TC level was higher in people with 1131T>C-CC genotype than other genotypes
in both groups (p=0.010, p=0.001). We determined that the odds ratio for the hypertriglyceridemia was
5.98 for ApoA5-1131T>C CC-genotype carriers.
- Full text:P020170421761079314737.pdf
