Study results on hepatoprotective effect of “antitoxic preparation” on test animal with acute liver infection developed by lipopolysaccharide
- VernacularTitle:Туршилтын амьтанд LPS-р үүсгэсэн элэгнийхурц хордлогот үрэвсэлд “Хордлого тайлах бэлдмэл”-н нөлөөг судалсан дүн
- Author:
Ulzii-Undrakh Ts
;
Uuganbayar B
;
Bolortulga Z
- Publication Type:Journal Article
- Keywords:
mung bean;
Soybean;
Antitoxic;
Antitoxic preparation
- From:Mongolian Medical Sciences
2015;172(2):104-109
- CountryMongolia
- Language:Mongolian
-
Abstract:
Introduction
The use of phytochemical preparations is being promoted an supported worldwide. In order
to investigate and confirm the usage of phytochemical preparations that are widely used in the
traditional medicine, there is an urgent need to complete a chemical, phytochemical and clinical
study for those medicinal preparations.
Goal
To investigate the effects of “Antitoxic preparation” on the test animal with previously developed acute
hepatotoxic infection by LPS.
Materials and Methods
A pathology model of the acute liver infection was developed on a total of 50 Vister rats, weighing
between 200 and 250 gr. The test animals were categorized info five five further groups, e.g. healthy,
control, comparison and administered with 50 mg/kg and 100 mg/kg of “Antitoxic preparation”. The
preparation for each group was individually and orally administeredfor a period of ten days. On day
11, 2nd, 3rd, 4th and 5th groups were administered 5 μg of LPS and 300 mg/kg of GaIN calculated in
2 ml of physiogial solution was injected in the abdomed of the test animal. After 8 hours, AST, ALT,
cholesterol, triglycerides, level of MDA, cytokine levels such as TNF-α, IL-1β, IL-6, IL-10 contained in
the blood plasma of test animals were analysed.
Results
A comparison between measurement of “Antitoxic preparation” group and control group has indicated
that the AST was 24.9-30.8%, ALT 23.8-27.6%, Poenzyme activation was reduced by 29.1-32.6%, of
cholesterol by 13.2-19.9%, of tryglyceride 23.4-30.5%, MDA in plasma 8.8-20.9%, MDA in urine 11.3-
22.9%, also reduction of TNF-α in plasma by 17.5-27.3% and IL-1β17.7-19.8% respectively. Also,
it was determined that the cytokines activating the acute liver infection (TNF-α, IL-1β, IL-10) were
impacted after administering the preparation and infection process was suppressed.
Conclusions:
1. The pathological model for chronic toxic liver infection developed on the test animal indicated
that “Antitoxic preparation” had a reducing effect on cholesterol, tryglycerides, inhibitory
effect on activation of fat oxidation, choleretic, antioxidant, reducing effect on ALT, AST
activation, reducing the destruction of liver cells and followed by hepatoprotective action.
2. “Antitoxic preparation” was effective in impacting the cytokines (TNF-α, IL-1β, IL-10) that
activate the acute liver infection and also suppressing effect on infection process.
- Full text:P020170421594109622481.pdf
