The detection of adherence factorsby Escherichia coli cause of urinary tract infectionsin Ulaanbaatar, Mongolia
- VernacularTitle:Шээсний замын халдвар үүсгэгч Escherichia coli-ийн адгезийн хоруу шинжийг илрүүлсэн дүн
- Author:
Munkhdelger Ya
;
Davaasuren S
;
Dolgorjav B
;
Gerelee A
;
Oyunchimeg R
;
Sarantuya J
- Publication Type:Journal Article
- Keywords:
biofilm formation;
cell surface protein (curli);
UPEC (uropathogenic E.coli)
- From:Mongolian Medical Sciences
2014;168(2):5-8
- CountryMongolia
- Language:Mongolian
-
Abstract:
INTRODUCTION:
Urinary tract infections among the most common bacterial infectious diseases encountered at all
ages. Escherichia coli are being the etiologic agent in 50–80%. Therefore, it is an important public
health problem. E.coli causing urinary tract infections express pilli, fimbriae and others adherence
virulence factors.
GOAL:
To detect the some adherence virulence factors of Uropathogenic Escherichia coli (UPEC) in
Ulaanbaatar, Mongolia
MATERIALS AND METHODS:
A total of 76E.colisampleswere collected. These samples were positive bacteriological examination
of urine, performed at the bacteriological laboratory of the State Central Third Hospital and State
Central First Hospital, Ulaanbaatar, Mongolia. The biofilm formation was evaluated by the growth
rate of E.coli on plastic surface.The detection of the virulence factors type 1 fimbriae (fimA gene) and
P-fimbriae (papC) was performed by multiplex PCR using gene specific primers.Curli expression
was determined by using congo red agar.
RESULTS:
The evaluation of bacterial biofilm formation using 96 well plates showed 40 negative (52.6%),
32 weak biofilm (42.1%) and 4 moderate biofilm (5.3%) formation for E.coli and no strong biofilm
forming strain was detected. The cell surface protein (curli) was detected by Congo red agar. The
result was 71% positive for studied E.coli strains. The detection result of pili genes by multiplex
PCR showed that fimH gene detected for 73 (96.1%) and papC gene detected for 18 (23.7%) E.coli
cultures.
CONCLUSION: Almost half of surveyed Uropathogenic E.coli isolated in Ulaanbaatar, Mongolia had
ability of biofilm formation and it has been determined by the bacterial surface protein (curli), which
is one of bacterial adherence factors, may cause biofilm formation.
- Full text:P020151216482150972281.pdf
