The oral microbiome community variations associated with normal, potentially malignant disorders and malignant lesions of the oral cavity
- Author:
MOK Shao Feng
;
YAP Sook Fan
;
Alan ONG Han Kiat
- Publication Type:Journal Article
- Keywords:
oral bacteria, oral cancer, Malaysia, 16S cloned library, DGGE
- From:The Malaysian Journal of Pathology
2017;39(1):1-15
- CountryMalaysia
- Language:English
-
Abstract:
The human oral microbiome has been known to show strong association with various oral diseases
including oral cancer. This study attempts to characterize the community variations between
normal, oral potentially malignant disorders (OPMD) and cancer associated microbiota using 16S
rDNA sequencing. Swab samples were collected from three groups (normal, OPMD and oral
cancer) with nine subjects from each group. Bacteria genomic DNA was isolated in which full
length 16S rDNA were amplified and used for cloned library sequencing. 16S rDNA sequences
were processed and analysed with MOTHUR. A core oral microbiome was identified consisting
of Firmicutes, Proteobacteria, Fusobacteria, Bacteroidetes and Actinobacteria at the phylum level
while Streptococcus, Veillonella, Gemella, Granulicatella, Neisseria, Haemophilus, Selenomonas,
Fusobacterium, Leptotrichia, Prevotella, Porphyromonas and Lachnoanaerobaculum were detected at
the genus level. Firmicutes and Streptococcus were the predominant phylum and genus respectively.
Potential oral microbiome memberships unique to normal, OPMD and oral cancer oral cavities were
also identified. Analysis of Molecular Variance (AMOVA) showed a significant difference between
the normal and the cancer associated oral microbiota but not between the OPMD and the other two
groups. However, 2D NMDS showed an overlapping of the OPMD associated oral microbiome
between the normal and cancer groups. These findings indicated that oral microbes could be potential
biomarkers to distinguish between normal, OPMD and cancer subjects.
- Full text:P020170515565130719930.pdf
