Changes in Corpus Cavernosum after Partial Bladder Outlet Obstruction in Rat.
10.4111/kju.2008.49.2.160
- Author:
Kyo Ik MO
1
;
Hyung Il LEE
;
Kyung Seop LEE
Author Information
1. Department of Urology, College of Medicine, Dongguk University, Gyeongju, Korea. ksleemd@ dongguk.ac.kr
- Publication Type:Original Article
- Keywords:
Partial bladder outlet obstruction;
Endothelin-1;
Endothelial nitric oxide synthase;
Vascular endothelial growth factor;
Apoptosis
- MeSH:
Animals;
Apoptosis;
Connective Tissue Cells;
Contracts;
Endothelin-1;
Erectile Dysfunction;
Humans;
Immunoblotting;
In Situ Nick-End Labeling;
Male;
Muscle, Smooth;
Neck;
Needles;
Nitric Oxide;
Nitric Oxide Synthase Type III;
Rats;
Rats, Sprague-Dawley;
Relaxation;
Sutures;
Urethra;
Urinary Bladder;
Urinary Bladder Neck Obstruction;
Vascular Endothelial Growth Factor A
- From:Korean Journal of Urology
2008;49(2):160-167
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Abnormalities of the relaxation and contraction of the corpus cavernosum can lead to erectile dysfunction. Therefore, we induced a partial bladder outlet obstruction(PBOO) in male rats, and investigated the mechanisms of penile dysfunction with endothelial nitric oxide synthase(eNOS), vascular endothelial growth factor(VEGF), endothelin-1(ET-1), and apoptosis of peri-vascular smooth muscle and connective tissue cells in the corpus cavernosum. MATERIALS AND METHODS: PBOO was induced in 13 Sprague-Dawley rats by placing a 25 gauge needle sheath around the urethra, then ligating the bladder neck with a 3-0 suture. Three week after surgery, distal penile tissues were dissected for immunohistochemical staining, immunoblotting, and TUNEL staining. RESULTS: The expression of eNOS and VEGF were significantly decreased, whereas the expression of ET-1 and apoptosis of perivascular smooth muscle and connective tissue cells were significantly increased in the corpus cavernosum. CONCLUSIONS: The significant increase of ET-1 and apoptosis along with decreased eNOS and VEGF could mediate erectile dysfunction.
