Burkholderia pseudomallei lectins: occurrence and expression
- Author:
Koh, S.F.
;
Tay, S.T.
;
Puthucheary, S.D
- Publication Type:Journal Article
- From:Tropical Biomedicine
2016;33(4):853-861
- CountryMalaysia
- Language:English
-
Abstract:
Lectins, also known as sugar binding proteins, play an essential role in the initiation
of bacterial infections and biofilm production. To date, several lectins of Gram-negative
bacteria such as Pseudomonas aeruginosa, Burkholderia cenocepacia, Ralstonia
solanacearum and Chromobacterium violaceum have been identified. There are no published
reports on the presence of lectins in Burkholderia pseudomallei, the causative agent of
melioidosis. The aim of this study was to identify possible lectin genes of B. pseudomallei and
generate recombinant proteins for assessment of hemagglutinating activity. Seven hypothetical
lectins of B. pseudomallei were retrieved from the UniProt database. Four lectin domains,
i.e., ricin B, C-type, H-type and Bulb-type lectins were identified. In silico analysis using a
ligand binding site prediction server (3DLigandSite) predicted the presence of Nacetylglucosamine
and calcium binding sites in two C-type lectins. Four recombinant proteins
with the molecular weights of 11.7, 30.2, 36.2 and 46.4 kDa were expressed from the cloned
genes; however none of them expressed any hemagglutinating activity. Further
characterization of B. pseudomallei lectins may be able to provide insights into bacterial-host
interaction that are required to initiate infections.
- Full text:P020170202534216602068.pdf
